ZFIN Image: Rissone et al., 2019, Fig. 5.

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Figure Caption

Fig. 5.

ak2 deficiency impairs IC neuromast formation. (A,B) Chemical treatments of ak2^hg14 mutants and their control siblings (WT) with AG1478 or 6-BIO. (A) Confocal analysis at 5 dpf of trunk regions for untreated, 0.03% DMSO or 3 µM AG1478-treated embryos in the Tg(tnks1bp1:EGFP; atoh1a:dTOM) transgenic background. Scale bars: 50 µm. (B) Alkaline phosphatase staining of 5 dpf ak2^hg14 and control embryos untreated or treated with 0.02% DMSO, 2 µM AG1478 or 0.2 µM 6-BIO compounds. White asterisks indicate IC neuromasts. Scale bars: 50 μm. (C) Quantification of the effect of AG1478 or 6-BIO treatments in inducing the formation of ectopic ICs in ak2^hg14 homozygous mutants and their control siblings. The total number of embryos analyzed per genotype is indicated above each bar.

Figure Data

Phenotype details

Acknowledgments

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