IMAGE

Fig. 5.

ID

ZDB-IMAGE-200116-13

Antibodies

Publication

Chagovetz et al., 2019 - Interactions among Ryanodine Receptor isotypes contribute to muscle fiber type development and function

All Figures

Figures for Chagovetz et al., 2019

Image

Figure Caption

Fig. 5.

RyR-mediated muscle contractions are required for slow fiber maturation. (A-I) Slow fibers were visualized with F59 antibody at 20 and 24 hpf and with the slow fiber-specific S58 antibody at 48 hpf. Slow fibers, which are initially wavy, mature and align with respect to each other as wild-type embryos develop. In ryr1b mutant embryos, maturation is delayed; in paralyzed triple mutants, fiber maturation is arrested. (J) Sample image indicating how slow muscle fiber (yellow) and A-P somite (magenta) lengths were determined using ImageJ. (K) Sample image of sarcomere banding indicating how sarcomere A-P lengths (white bracket) were determined. (L) Quantification of slow fiber:somite length ratios. (M) Quantification of sarcomere lengths of slow muscle fibers. One-way ANOVA was used to determine statistical relationships at each developmental time point with Tukey's multiple comparisons test used to adjust P-values. To determine fiber length or sarcomere length, five fibers were examined in each of five different embryos for each condition. ns, not significant.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.