Figure 5

Figure 5

Identification of ALK5 as a pkd2^−/− phenotype modulator in zebrafish. (a) Heat maps of known kinases inhibited by PKIS repressor compounds with the top 25 inhibited kinase targets in decreasing order. Numbers represent percentage of inhibition; dark yellow ≥65%, yellow ≥40%, light grey 35–40%, dark grey 30–35%, grey/blue 20–30%, light blue 15–20% and dark blue >15%. (b) Effects of potent KDR and (c) MAP4K4 inhibitors on pkd2^−/− zebrafish embryo tail curvature. All compounds tested at 10 µM. Results represented by mean ± SEM. No significance via one-way ANOVA and Dunnett’s multiple comparison. (d) Effects of different concentrations of the ALK5 inhibitor SD208 on pkd2^−/− curvature. Results represented by mean ± SEM. Significance via one-way ANOVA and Dunnett’s multiple comparison, p values represented by * (****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 and non-significant (ns): p > 0.05).