Figure 3

Figure 3

PKIS zebrafish screen revealed four PKIS repressors of the pkd2^−/− phenotype. (a) Hit compounds identified from the PKIS library after initial compound screening and validation. Enhancers of pkd2 curvature in red, repressors in green. Mean of DMSO baseline indicated with black line. Effect of re-ordered PKIS repressor compounds on pkd2 curvature (b) and glomerular area (c). Statistical significances by one-way ANOVA with Dunnett’s multiple comparison, results represented by mean +/− SEM. P values represented by * (****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 and non-significant (ns): p > 0.05.). (d) Chemical structure of the three validated PKIS tail curvature repressor compounds.