Fig. 6

Fig. 6

a Crystal structure of the SEA domain from zebrafish Gpr126. b (left) NMR structure of Mucin-1 SEA domain (PDB: 2ACM) and (right) Gpr126 SEA domain superimposed over Mucin-1 SEA domain. The loop containing the autoproteolysis site in Mucin-1 is indicated by a yellow arrow. The root-mean-square deviation (RMSD) of atoms between overlaid structures is 2.761 Å. c (left) Crystal structure of the Notch2 SEA domain (PDB: 2OO4) and (right) Gpr126 SEA domain superimposed over Notch2 SEA domain. The loop containing the furin-cleavage site (deleted in crystal structure construct) is indicated by an orange arrow. The RMSD of atoms between overlaid structures is 4.767 Å. d Sequence alignment of partial SEA domain from human Mucin-1, human Notch2, human GPR126, and zebrafish Gpr126. e Homology model of human GPR126 SEA model generated using SWISSMODEL. The arrow points to modelled furin-cleavage site. f Protein topology map of SEA domain. Furin-cleavage site is indicated by red scissors. Residues N-terminal to cleavage site are dark blue and residues C-terminal to cleavage site are light blue. Dashed lines represent backbone hydrogen bonds between beta sheets.