ZFIN Image: Osmani et al., 2019, Fig. 4

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Figure Caption

Fig. 4

ITGB1 but Not CD44 Is Required for Extravasation in the Zebrafish Embryo and Lung Metastasis in Mice

(A) D2A1 cells were transfected with the indicated siRNAs and microinjected into the duct of Cuvier of 2 dpf Tg(Fli1:EGFP) embryos. The cell localization pattern was qualified (B) and imaged (C) 24 h after injection.

(B) The ratio of cells extravasated (extravascular) over the total number of cells was measured.

(C) Representative images of cells at 24 hpi. The 3D rendering shows intravascular (blue) and extravascular (red) cells at 24 hpi.

(D) Scheme of the experimental approach for the mouse lung metastasis assay.

(E) Representative images of D2A1-Luciferase transfected with the indicated siRNAs in lung seeding (1 hpi) and metastatic outgrowth (13 dpi).

(F) Quantification of the luciferase signal of D2A1-luciferase (Luc) transfected with the indicated siRNAs in lung seeding (1 hpi, top panel) and metastatic outgrowth (13 dpi, bottom panel).

The graphs show the mean and minimum/maximum of 5 (B) or at least 2 (F) independent experiments.

Acknowledgments

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