ZFIN Image: Jiang et al., 2019, Fig. 1-S2

Image

Figure Caption

Fig. 1-S2

c4da neurons are enclosed by epidermal sheaths.

( A–C) Double labeling of sensory neurons (HRP) and epidermal sheaths (PLC^δ-PH-GFP fluorescence) stained under non-permeabilizing conditions. Maximum projection images show double labeling ( A), epidermal PLC^δ-PH-GFP signal intensity ( B), and HRP surface staining intensity ( C). Dashed lines mark sheath segments for which intensity profiles are shown in ( D, E). Note the apparent reciprocal relationship between surface-exposed HRP and PLC^δ-PH-GFP intensities. ( F, G) Scatterplots show the relationship between surface-exposed HRP and PLC^δ-PH-GFP signal intensities on sheaths from eight individual neurons at 84 h after egg laying (AEL), shortly after sheath formation is initiated ( F) and at 120 h AEL, when c4da neurons are extensively ensheathed ( G). Note the inverse linear regression.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife