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Thestrup et al., 2019 - A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation

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Fig. 5

EphB/EphrinB signaling controls HPD lumen morphogenesis independently of other morphogenetic roles. a Conditional ephrinb1^EC expression is induced by heat-shock prior to or during CBD remodeling, at 50 hpf or 54 hpf, respectively. Embryos were analysed at 60 hpf (heat-shock at 50 hpf: n = 7, heat-shock at 54 hpf: n = 7, both N = 1). b–d Hnf4α (green), Prox1 (magenta) and aPKC (gray) labeling show that unlike in controls (b), conditional ephrinb1^EC expression at 50 hpf (c) and 54 hpf (d) leads to HPD, liver and pancreas morphology defects, ectopic Prox1-positive cells throughout the HPD (blue arrowheads), with a subset associated with ectopic lumina bifurcating from the main duct (outlined with blue dashed line) disrupted and disorganized HPD lumen (red arrowheads, c, d). Yellow dotted lines: liver, yellow dashed line: pancreas; blue arrow: HPD system, orange arrow: CBD, green arrowhead: HPA

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