FIGURE 14

FIGURE 14

Low proliferative rate of mature HCS-cells during fin regeneration. (A) Immunofluorescent staining for SV2 (green) and PCNA (red) on sagittal section of fin wound epidermis at 3 dpa. (A’) SV2-positive cells are PCNA-negative (arrowheads). (B) Proportion of PCNA-positive cells among SV2-positive HCS-cells versus in SV2-negative epidermal cells (2.3% PCNA-positive in HCS-cells versus 13.8% PCNA-positive in other cells). N = 3. Number of cells for each group indicated on graph. (C) Experimental design for BrdU assays. (D,F) Immunofluorescence staining of whole mount fins at 3 dpa for serotonin (green) and BrdU (red) after 18 h or 48 h of BrdU-treatment. The image shows confocal imaging through the epidermis distal to the amputation plane (dashed line). (D’,F’) Zoomed areas show BrdU-negative (white arrowhead) and BrdU-positive (yellow arrowhead) 5-HT-positive cells. (E,G) Proportion of BrdU-positive cells among 5-HT-positive cells after 18 and 48 h of labeling (5.4 and 71%, respectively). N = 3 fins; total number of cells in each group indicated on graph.