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Publication: Giallongo et al., 2019 - TLR4 signaling drives mesenchymal stromal cells commitment to promote tumor microenvironment transformation in multiple myeloma
All Figures: Figures for Giallongo et al., 2019

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Fig. 1 SMM- and MM-MSC activated neutrophils in immunosuppressive and pro-angiogenic cells.

a Only SMM- and MM-MSCed-N exhibited suppressive effects compared to N control (isolated from PBMC cultured without MSC). CTRL+ : T lymphocytes incubated only with PHA. b Before incubation with T cells, ed-N were analyzed for the expression of immune modulatory factors. Calculated value of 2^−ΔΔCt in control (HC-MSC educated-neutrophils) was 1. c Adding BTZ, LENA or POMA during co-culture of PBMC with MM-MSC, isolated neutrophils did not lose immunosuppressive ability. Ref MM-MSC: MSC isolated from patients with refractory MM. d HMEC were plated on Matrigel in the absence (1, control) or presence of VEGF-A (2, positive control), of SMM-MSCed-N (3), MM-MSCed-N (4), MM-MSCed-N isolated from co-culture with BTZ (5) or LENA (6), refractory MM-MSCed-N (7) or refractory MM-MSCed-N isolated from co-culture with POMA (8). After 5 h, SMM-MSCed-N and refractory MM- and MM-MSCed-N induced tube formation. The pro-angiogenic effect was significantly reduced by the proteasome inhibitor and the immunomodulatory drugs. *p < 0.05; **p < 0.01; ***p < 0.001

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