FIGURE 2

FIGURE 2

Molecular markers of HCS-cells in the adult fin epidermis. (A–C) Immunofluorescence staining of longitudinal sections of uninjured adult caudal fins; ep, epidermis, mes, mesenchyme. Dashed lines indicate the border between epidermis and mesenchyme. (A,A’) Rabbit antibody against serotonin (5-HT; green) and mouse antibody against Synaptic vesicle glycoprotein 2 (SV2; red) colocalize in single cells near the epidermal surface. (A’) A higher magnification of the framed area in panel (A) shows the vesicular and polarized distribution of both markers in the cells. (B–B”) Triple immunostaining with rat antibody against serotonin (5-HT), rabbit antibody against Calretinin and mouse antibody against Synaptic vesicle glycoprotein 2. All three markers are expressed in the same cells of the epidermis. Serotonin and SV2 are polarized while Calretinin is found throughout the cytoplasm. (C,C’) Mouse antibody against serotonin and rabbit antibody against Calretinin label the same cells. All three serotonin antibodies have identical patterns. However, both Rat-serotonin and Mouse-serotonin required high concentrations and gave weaker signals than the rabbit antibody. N ≥ 4 for each staining. Nuclei are labeled with DAPI (blue). (A’,B’,C’) Images labeled with letters with prime symbols show higher magnifications of the frames in the corresponding images. The same rule applies to all the subsequent figures.