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Fig. 1 S1

ID: ZDB-IMAGE-191230-606
Publication: Rahman et al., 2019 - Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
All Figures: Figures for Rahman et al., 2019

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Figure Caption

Fig. 1 S1 Schematics showing PKIS screen design.

(A) Tail fin transected three dpf Tg(mpx:GFP)i114 zebrafish larvae that had generated an inflammatory response at six hpi were incubated with individual PKIS compounds [25 µM] for a further 6 hr. Larvae were imaged and manually scored between 0–3 on the basis of green fluorescence at the injury site. (B) PKIS compounds were incubated with primary human neutrophils for 6 hr. Apoptosis was assessed by Annexin V/TO-PRO-3 staining by flow cytometry and the percentage apoptosis calculated as Annexin V single plus Annexin V/TO-PRO-3 dual positive events (as indicated by red box).

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