Pramipexole (PPX) enhances zebrafish regulatory T cell (zTreg) recruitment in regenerating tail tissue. (a) Regenerating tissue from DMSO- and PPX-treated zebrafish at 3 days post-injury (dpi). White dotted lines represent the amputation line. Cells distal to this line were counted. (b) Dose–response for PPX (0.5–20 μM) in the tail amputation assay. Quantification of foxp3a+ cells (mean ± SEM). (c) Quantification of foxp3a+ cells (mean ± SEM) in uninjured tail tissue from zebrafish treated with PPX or DMSO for 3 days. n.s., non-significant; * p < 0.05, ** p < 0.001. Number of animals in each group indicated in [].
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