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Figure 1
Membrane integrity after vitrification. V1 (1.5 M methanol + 5.5 M Me2SO + 0.5 M sucrose); V2 (1.5 M methanol + 5.5 M Me2SO + 0.5 M sucrose + 10% egg yolk solution); V3 (1.5 M methanol + 5.5 M Me2SO + 0.5 M trehalose); SV4 (1.5 M methanol + 5.5 M Me2SO + 0.5 M trehalose + 10% egg yolk solution). Control = fresh ovarian tissue fragments. Mean ± SE followed by different letters differ by Tukey’s test (P = 0.0004).
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