FIG 1

FIG 1

MMARE11_28540 is required for the processing of LipY. (A) Secretion of LipY-OVA₂ by M. marinum wild-type E11, mmarE11_28540::tr, and complemented strains was detected by double filter assay. (B) Alignment between model of MMARE11_28540 (red, amino acids 269 to 551 out of 554) and crystal structure of PE_PGRS16 (blue, 4EHC). Aspartic acids within the putative active site DTG/DSG motifs are depicted as spheres. (C) Immunoblot analysis of whole-cell lysate from M. marinum M wild-type and Δmmar_2933 strains expressing C-terminally HA-labeled LipY_tub or LipY_A150D. LipY was detected with anti-HA antibody. (D) The cleavage sites of MMAR_2933 in the linker domain of LipY_tub as determined by N-terminal Edman sequencing; the N-terminal cleavage site (aa 136A to 137A) and the previously identified C-terminal cleavage (aa 149G to 150A) (23) in LipY_tub are indicated. (E) Immunoblot analysis of whole-cell lysate from M. marinum M wild-type and Δmmar_2933 strains expressing C-terminally HA-labeled LipY_Δ98-201. Proteins were detected with anti-HA antibody.