FO promotes caudal fin regeneration in adult zebrafish. Adult zebrafish (n = 15) were immersed in FO for 12 days post amputation (dpa). Next, fish caudal fins were subjected to double fluorochrome labeling with calcein and alizarin red at 6 and 12 dpa, respectively. Bone regions labeled with calcein (green) and alizarin red (red) indicate new bone regeneration at 6 and 12 dpa. Relative intensity of each bone vein was calculated using the Image J software. Amputation axis is also indicated (dashed line). Significant differences among the groups were determined using the one-way ANOVA followed by Bonferroni correction. All data are presented as mean ± SEM (*** p < 0.001, ** p < 0.01, and * p < 0.05 versus untreated group). FO; fermented extract of C. gigas and GP; β-glycerophosphate.
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