Figure 3

Figure 3

FO upregulates ALP expression and activity in preosteoblast MC3T3-E1 cells. (A) MC3T3-E1 cells were treated with FO (50 µg/mL and 100 µg/mL) or DEX (100 nM) for 7 days. Representative images from three biological replicates were collected using the Toup View software after staining the cell for ALP activity (as described in the Materials and Methods section) on day 3 (D3), day 5 (D5), and day 7 (D7). (B) The relative ALP staining density was represented using mean integrated pixel density. (C) ALP activity was measured at 520 nm using a tartrate-resistant acid phosphatase (TRACP) & alkaline phosphatase (ALP) assay kit. Significant differences among the groups were determined using the one-way ANOVA followed by Bonferroni correction. All data are presented as mean ± SEM (*** p < 0.001 and * p < 0.05 versus untreated group). FO; fermented extract of C. gigas and DEX; dexamethasone.