IMAGE

Figure 5-figure supplement 3.

ID: ZDB-IMAGE-191230-1828
Publication: O'Brown et al., 2019 - Suppression of transcytosis regulates zebrafish blood-brain barrier function
All Figures: Figures for O'Brown et al., 2019

Image

Figure Caption

Figure 5-figure supplement 3. <italic>Mfsd2aahm37/hm37; mfsd2abhm38/hm38</italic> double mutants display similar increased BBB permeability to <italic>mfsd2aahm37/hm37</italic> single mutants.

(A–D) Representative maximum intensity projection images of the midbrain of wild-type (A; mfsd2aa^+/+; mfsd2ab^+/+), mfsd2aa^hm37/hm37 single mutants (B), mfsd2ab^hm38/hm38 single mutants (C), and mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants (D) expressing the fluorescently labeled 80 kDa transgenic serum protein DBP-EGFP (green) at 5 dpf. Mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants display a similar level of increased parenchymal tracer permeability to the mfsd2aa^hm37/hm37 single mutants. The scale bar represents 50 µm. (E) Quantification of DBP-EGFP tracer intensity in the midbrain parenchyma of wild-type (black), mfsd2aa^hm37/hm37 mutants (magenta), mfsd2ab^hm38/hm38 mutants (green), and mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants (turquoise). Each individual fish measured is displayed as a single point. The mean and the standard error are drawn in black. ****p<0.0001 by two-way ANOVA.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife