Figure 1

Figure 1

Loss of Nup62l led to severely impaired formation of PA cartilages. (A) Diagram showing the CRISRP/Cas9 target DNA sequence (underline) of zebrafish gene nup62l. PAM region (AGG) was shown in red. (B) Sanger sequencing results revealed an 11-bp genomic DNA fragment insertion at the target site in nup62l-mutants. (C) Lateral views showing the pharyngeal morphology of WT siblings. Embryos of nup62l-mutants at 60 hpf or 96 hpf were injected with or without 300 pg nup62l mRNA/embryo. White lines indicated the pharyngeal regions. Note a shortened and thinned pharyngeal region in nup62l-mutants. (D) Images showing pharyngeal cartilage elements stained with alcian blue in WT. The nup62l-mutants injected with or without 300 pg nup62l mRNA at 96 hpf were ventrally viewed in upper panels and laterally viewed in lower panels. cbs, ceratobranchials; ch, ceratohyal; ep, ethmoid plate; hs, hyosymplectic; m, Meckel’s cartilage; no, notochord; pc, parachordal; pq, palatoquadrate; tr, trabecula.