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Figure 5—figure supplement 1.

ID: ZDB-IMAGE-191230-1601
Source: Figures for Young et al., 2019

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Figure 5—figure supplement 1. 4L-Tcf7l2 and 45L-Tcf7l2 variants interact with β-Catenin and Tle3b in yeast two-hybrid protein interaction assays.

Y2Gold yeast strain was co-transformed with: (1) β-catenin/pGAD and empty pGBK vector, (2) β-catenin/pGAD and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) (3) empty pGAD vector and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) with, (4) dCtle3b/pGAD and Empty pGBK vector, (5) dCtle3b/pGAD and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) (6) empty pGBK and pGAD vectors. and plated in -Leu-Trp dropout selective media agar plates supplemented with Xgal (A, C) or Ade-His-Leu-Trp dropout selective media agar plates supplemented with Aureoblastidin A and X-gal (B, D).

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