IMAGE

Figure 4.

ID: ZDB-IMAGE-191230-1599
Publication: Young et al., 2019 - Developmentally regulated Tcf7l2 splice variants mediate transcriptional repressor functions during eye formation
All Figures: Figures for Young et al., 2019

Image

Figure Caption

Figure 4. Exon five coding <italic>tcf7l2</italic> variant represses Wnt activity induced by <italic>FLAG-Ax2</italic> or <italic>VP16-TCF7L2</italic> in luciferase assays.

Bar plots showing luciferase reporter assay results expressed in relative light units. HEK293 cells were transiently co-transfected with luciferase reporter constructs indicated beneath the X-axis, (A–F) FLAG-Ax2 (except for first bars), (G–L) VP16-TCF7L2 DNA (except for first bars) and 4L-tcf7l2 DNA (+4L; 3^rd bars), or 45L-tcf7l2 DNA (+45L; 4^th bars) or tcf7l2-AA DNA (+AA; 5^th bars). Control experiments show only background luciferase activity with no transfected plasmids (1^st bars). Figures in the bars indicate the percentage size of that bar relative to transfection with either FLAG-Ax2 or VP16-TCF7L2 alone (2^nd bar). Error bars are mean ± SD n = 3 (experiments were performed twice), P values from unpaired t tests comparing FLAG-Ax2 or VP16- TCF7L2 control condition with tcf7l2 variant co-transfections. Comparisons with no statistical significance are not marked.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife