ZFIN Image: Morishita et al., 2019, Figure 6

Image

Figure Caption

Figure 6 Depletion of VMP1 in HepG2 cells does not affect proteasome activity, ER stress, and MTTP expression.

(A) HepG2 cells were treated with siRNA oligonucleotides against luciferase (Luc) or VMP1 and cultured in regular medium in the presence or absence of 5 μM lactacystin for 24 hr. Chymotrypsin-like proteasome activity was measured. Data represent the mean ± standard error of the mean (n = 3) and were statistically analyzed using the Student’s t-test. (B and C) HepG2 cells were treated with siRNA oligonucleotides as in (A) and cultured in regular medium in the presence or absence of 200 nM oleic acid for 24 hr. Cell lysates were analyzed by immunoblotting.

10.7554/eLife.48834.017Figure 6—figure supplement 1—source data 1.Related to <xref rid='fig6s1' ref-type='fig'>Figure 6—figure supplement 1A</xref>.

Acknowledgments

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