Figure 4—figure supplement 1.

Figure 4—figure supplement 1.

(a,b) Representative confocal images of mid-trunk of 3 dpf of (a) Tg(mrc1a:eGFP) or (b) Tg(mrc1a:mir204-eGFP). The thoracic duct is pseudocolored in green, with other vessels in gray. (c) Quantification of thoracic duct formation in animals as in panels a and b. The number of somitic segments with an intact thoracic duct was counted, with the same seven mid-trunk somites measured in each animal. (d) Quantification of the area between the dorsal aorta (DA) and posterior cardinal vein (PCV) in animals as in panels a and b, as an indicator of developmental timing. (e) Quantitation of mir-204 level measured by TaqMan qPCR from whole embryo at three dpf. All graphs are analyzed by t-test and the mean ± SD is shown.