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Figure 8

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ZDB-IMAGE-191230-1507

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Collodet et al., 2019 - AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR

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Figure 8

Activation of AMPK leads to translocation of TFEB to the nucleus and increased expression of flcn and fnip2 in zebrafish. A) Embryos at 3 dpf were treated with 991 10 µM or vehicle (DMSO) for 16 h. Tissue lysates (20 µg) were resolved by SDS-PAGE and Western blot analysis was performed using the indicated antibodies. B) Tg(actc1b:tfeb-ZsGreen);Tg(actc1b:nls-mCherry) embryos at 3 dpf were treated with 991 10 µM or vehicle (DMSO) for 24 h. Embryos were mounted live in water containing 0.016% tricaine and imaged with ImageXpress confocal system at original magnification, ×20, the scale bar corresponds to 50 µm (n = 12). C) Schematic summary of the acute exercise study design using WT and prkaa1^−/−;prkaa2^−/− zebrafish. D) Immediately after the 3-h training session, muscle samples were collected from the zebrafish. Tissue lysates (20 µg) were resolved by SDS-PAGE and Western blot analysis was performed using the indicated antibodies. E) Relative mRNA transcript levels of flcn and fnip2 were determined by qPCR. Data are shown as box-and-whisker plots (minimum to maximum) of values normalized to control WT (n = 6). Two-way ANOVA with the factors of genetic background and exercise, plus the interaction between these 2 factors, was performed. The graph shows the significance of the interaction. *P < 0.05, ****P < 0.0001.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.