Fig 6

Fig 6

(A,B) Relative frequency of shuttles for different cargos, incidence computed for each condition as number of 3-h imaging datasets with shuttle(s)/total number of imaging datasets. By chi-squared analysis, there are no significant differences for the comparisons: live spores of the two species (p = 0.38), live and dead T. marneffei (p = 0.31), and dead spores of the two species (p = 0.34). n-values indicate the number of datasets in each category. (C,D) Images of representative shuttles of zymosan particle (C) and β-glucan–coated plastic beads (D). Shuttles of particles (blue) are from Tg(mpx:EGFP) neutrophils (green) to Tg(mpeg1:Gal4FF)×(UAS-E1b:Eco.NfsB-mCherry) macrophages (red). In each example, panels include isometric orthogonal yz and xz views corresponding to the xy maximal intensity projection and indicate the time in min from start of movie. Colored arrowheads indicate the conidium within donor neutrophil (green), at the point of intercellular transfer (white) and in the recipient macrophage (red). Scales as shown. Stills in C, D correspond to S5A and S5C Movie, respectively. Datasets for A–B are provided in S1 Data. Eco.Nfsb, E. coli nitroreductase; EGFP, enhanced green fluorescent protein; Gal4FF, engineered form of S. cerevisiae Gal4 transcriptional activator; mpeg1, macrophage-expressed gene 1; mpx, myeloid-specific peroxidase; Tg, transgenic; UAS-E1b, upstream activating sequence fused to minimal adenovirus E1b promoter.