Figure 1

Figure 1

A. hydrophila-induced increased mortality in copper-stressed larvae. (A)A. hydrophila used in this study induced increased expression of immune-related genes in zebrafish larvae at 6 hpi (hours post infection), with increased expression observed in il-6, TLR signaling genes, MAPK signaling genes, stat1a, c3a, defb1, and hsp70.3 after only immersion infection with A. hydrophila. (B) Larvae were immersed with a different dosage of A. hydrophila at 68 hpf (hours post fertilization), and cumulative mortalities of the no-copper-stressed control and the copper-stressed larvae were calculated separately at 6, 12, 24, 36, 48, 60, and 72 hpi. (C) Larvae were immersed only or immersed after injury with 10⁷A. hydrophila at 68 hpf, and cumulative mortalities of the no-copper-stressed control and copper-stressed larvae were calculated separately at 6, 12, 24, 36, 48, 60, and 72 hpi. Three biological replicates were performed. ANOVA post-hoc Tukey's test. Data are presented as mean ± SD. ***P < 0.001; **P < 0.01; *P < 0.05.