FIGURE 2

FIGURE 2

Germ-free zebrafish colonized with GFP labeled Bacillus subtilis WB800N. (A) Fluorescence images of germ-free (GF) zebrafish and germ-free (GF) zebrafish mono-associated with different concentrations of B. subtilis WB800N (10², 10⁴, 10⁵, 10⁶CFU/ml). The magnification of the fluorescence microscope is 3.5×. (B) The survival rate of germ-free (GF) zebrafish, conventional raised (CONR) zebrafish and germ-free zebrafish exposed to different concentrations of B. subtilis WB800N at 3 dpf for 24 or 48 h. (C) The survival rate of GF zebrafish, conventionally raised (CONR) zebrafish and germ-free zebrafish exposed to different concentrations of B. subtilis WB800N at 5 dpf for 24 or 48 h. (D) Colonization concentrations (CFU/fish) of mono-association zebrafish colonized at 3 dpf for 24 or 48 h. (E) Colonization concentrations (CFU/fish) of GF zebrafish at 5 dpf for 24 or 48 h. All data (B–E) are presented as means ± SEM, n = 45 per group.