FIGURE 1

FIGURE 1

Germ-free zebrafish colonized with GFP labeled Escherichia coli DH5α. (A) Fluorescence images of GF zebrafish and GF zebrafish mono-associated with 10², 10⁴, 10⁵, 10⁶ CFU/ml E. coli DH5α at 3 dpf for 24 h. The magnification of the fluorescence microscope is 3.5×. (B) The survival rate of germ-free (GF) zebrafish, conventional raised (CONR) zebrafish and germ-free zebrafish exposed to different concentrations of E. coli DH5α at 3 dpf for 24 or 48 h. (C) The survival rate of germ-free (GF) zebrafish, conventionally raised (CONR) zebrafish and germ-free zebrafish exposed to different concentrations of E. coli DH5α at 5 dpf for 24 or 48 h. (D) Colonization concentrations (CFU/fish) of mono-associated zebrafish colonized at 3 dpf for 24 or 48 h. (E) Colonization concentrations (CFU/fish) of mono-associated zebrafish at 5 dpf for 24 or 48 h. All data (B–E) are presented as means ± SEM, n = 45 per group.