ZFIN Image: Kefalos et al., 2019, Figure 3

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Figure Caption

Figure 3

Notch signaling is activated during valve regeneration. (A) Confocal z-stack of a Tg(TP1:VenusPEST)/hspGFFDMC73A: UAS-E1b:NfsB-mCherry double transgenic embryo at 5 dpf. Tg(TP1:VenusPEST) signal of activated Notch signaling is restricted to the valves. Scale bar: 100 microns. (B) 1 day post MTZ treatments. Expansion of activation of Notch signaling throughout the ventricle is observed (blue arrows) Scale bar: 50microns. Confocal z-stacks of untreated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (C) and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (F) double transgenic embryos at 6 dpf. Confocal z-stack of MTZ treated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (D), and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (G), at 6 dpf. Confocal z-stack of MTZ treated and then during regeneration 48 hours DAPT treated Tg(hspGFFDMC73A: UAS-E1b:NfsB-mCherry) (E), and Tg(GSAIzGFFD703A: UAS-E1b:NfsB-mCherry) (H), at 6 dpf. (I) Each experiment was carried out at least 3 independent times with n = 10 embryos per experimental group. Scale bars: 20 micron.

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