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Figure 6—figure supplement 5. Loss of lymphatic vessels does not effect scar formation after amputation injury.

AFOG-histology images showing fibrin in red and collagen in blue in developmentally heat shocked (HS; 35 to 206dpf; B, C, E, F) or non-heat shocked (nonHS; A, D) non-transgenic (nonTg; A, B, D, E) and transgenic (hsp70l:sflt4; sFlt4; C and F) zebrafish 30 days (237dpf) following apex resection injury (30dpa, 8mpf). In all conditions at 30dpa, some hearts show little or no scar tissue (A-C) while other hearts of the same batch have detectable scar tissue still (D-F). (G) Graph showing the non-significant distribution (ns, Chi-Squared) of the presence or absence of scar tissue (examples images, A-F) between non-transgenic control (both nonHS, n = 6, and HS, n = 5) and transgenic (hsp70l:sflt4, n = 7) zebrafish at 30dpa. Scale bars 200 μm.

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