FIGURE 7

FIGURE 7

Expression of GDNF in the intestinal mesenchyme requires BMP2 signaling. (A,a,b) control embryos(97/103, 94.2%), (A,c,d) BMP 2b MO treated embryos) lateral views of 72 hpf whole mount embryos(112/124, 90.3%) that have been hybridized with a gdnf antisense probe. Compared with the control group, there is only partial expression of GDNF at the anterior end of the intestine at this age. (A,e,f) BMP2b MO plus heat-shocked at 10 hpf(84/105, 80%), the expression of GDNF was partly rescued. (A,j,h) BMP2b MO plus BMP2b mRNA were co-injected, the expression of GDNF was partly rescued(84/105, 80%). (B) The intensity of GDNF expression in different group. The expression of GDNF mRNA in the BMP2b MO group is approximately 2.7 fold the intensity of BMP2b MO + HS group. The expression of GDNF mRNA in the BMP2b MO group is approximately 2.5 fold the intensity of BMP2b MO + BMP2b mRNA group. Values are given as mean ± SE. n = 6, t-test, ^∗P < 0.05, compared with BMP2b MO + HS group or BMP2b MO + BMP2b mRNA group.) (C) Real time PCR analysis of GDNF mRNA expression. The GDNF mRNA expression was rescued in BMP2b + HS group, n = 6, t-test, ^∗P < 0.05, compared with BMP2b MO group. Bar, 100 μm.