FIGURE 2

FIGURE 2

Abnormal expression of wipi1 affected zebrafish embryo convergent extension. (A) Zebrafish embryos injected with wipi1-MO (antisense morpholino) show defects of convergent extension, including shortening of the trunk, and crooked or bent tails. To assess the extension defects quantitatively, the phenotypic classes were scored as grade 1 (normal), grade 2 (mild), grade 3 (moderate), and grade 4 (severe) in zebrafish embryos at 24 and 48 h post-fertilization (hpf) (scale bar = 500 μm). (B) Zebrafish embryos injected with wipi1-MO exhibit dosage-dependent convergent extension deficits at 24 and 48 hpf. Injection with wipi1-MO at dose of 2–6 ng showed a significant difference compared with control injection. (C) Overexpression of Wipi1 in zebrafish embryos at 24 and 48 hpf. Injection 200 pg mRNA and 300 pg mRNA resulted in a significant increase of malformed zebrafish. There was no correlation between mRNA dosage and severity of malformation (^∗P < 0.05).