Figure 8

Figure 8

The locomotor activity of zPnpo morphants at 4 dpf. Zebrafish larvae with the indicated treatment were placed individually in each well on a 48-well plate and rested in the tested chamber for 5 min before recording. (A) The swimming track, representing larval cumulative location in 1 min, was recorded. (B–E) Larval swimming behavior was recorded for 30 min and analyzed for velocity (B), maximum acceleration (C), body activity (D), and absolute turning angle (E) with the built-in analytic software in DanioVision High-throughput tracking system. Reported are the averages of data from at least three independent repeats with n = 30∼164 embryos for each group and expressed as mean ± SEM. The statistical significance was calculated with one-way ANOVA (Kruskal–Wallis test) by comparing the experimental groups with wild type (*) or with zPnpo MO (#). ^#p < 0.05; ^##p < 0.01; ***/^###p < 0.001. MO, morpholino oligonucleotide; ANOVA, analysis of variance.