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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-191230-102
Source: Figures for Harrison et al., 2019

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Figure Caption

Figure 1—figure supplement 1. Expression of lymphatic endothelial marker genes in the cardiac lymphatic system.

Whole-mount confocal imaging of transgenic zebrafish hearts expressing the pan-endothelial fli1a:GFP (A and B, Green), venous/lymphatic flt4:mCitrine (A and B, Blue), lymphatic makers prox1:Gal4-UAS:RFP (A and B, Red), lyve1:eGFP (C, Green), lyve1:DsRed (D, Red), stab1:YFP (E, Yellow), mrc1a:eGFP (F, Green), blood endothelial marker kdrl:mCherry (F, Red). (A and B) In more mature zebrafish the cardiac lymphatic vessel extends the entire length of the ventricle expressing both prox1:Gal4-UAS:RFP and flt4:mCitrine its entire length. The Cardiac lymphatic endothelium expresses lyve1:eGFP/DsRed (C and D), stab1:YFP (E) and mrc1a:eGFP (F). Unlike blood endothelial cells, these lymphatic endothelial cells completely lack expression of kdrl:mCherry (F). Scale bars 200 μm (A) and 50 μm (B-F).

Acknowledgments

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