ZFIN Image: Ayala-Nunez et al., 2019, Fig. 9

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Figure Caption

Fig. 9

Transmigrated ZIKV-infected monocytes promote viral dissemination to cerebral organoids. a Representative scheme of the experimental design. Neural organoids were located under a transwell where hCMEC/D3 cells were previously grown for 7 days. On top of a transwell, ZIKV-infected monocytes from two healthy donors, not-infected monocytes, cell-free ZIKV, or mock were added. After an overnight incubation, the transwells were removed and the organoids were cultured for 2 or 9 days, and then they were fixed and processed for b immunofluorescence or c flow cytometry. The bar graph shows the mean of an experiment performed in duplicate ± SD. Two-tailed p value < 0.05 (*). Statistical significance was determined by using a t test. Scale bar: 50 µm. Source data in c are provided as a Source Data file

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