Fig. 7

Fig. 7

ZIKV enhances monocyte transmigration in zebrafish embryos. a Representative scheme of the experimental design associated with the zebrafish model. Human primary monocytes were noninfected (NI), or infected for 48 h with ZIKV in the absence (ZIKV) or presence (ADE–ZIKV) of 20 ng/mL of the 4G2-enhancing pan-Flaviviridae antibody (see Supplementary Fig. 10), stained with CellTrace, and injected into the duct of Cuvier of Tg(fli1a:eGFP) zebrafish embryos (GFP-labeled endothelial cells⁴⁴). b Zebrafish imaging was done at 6–8 h post injection by scanning confocal microscopy. Representative 3D confocal image of the monocytes’ distribution within the tail of a zebrafish embryo vasculature (associated with Supplementary Movie 1) at 6 h post injection. Scale bar: 40 µm. c Three-dimensional reconstruction of the endothelium (green) and monocytes (magenta) that remained in the bloodstream (left panel, intravascular), transmigrated (middle panel, extravascular), or in the process of transmigrating (right panel). Scale bar: 25 µm. d Cell dispersion was manually counted and localized in the caudal plexus by using the stereotype patterning of intersegmental vessels (ISVs) as a reference. The data were compiled to generate heatmaps by using a custom-made MATLAB plugin. Representative heatmaps are represented for fish injected with monocytes from three individual donors. e Quantification of the mean ± SD of the ratio of extravasated monocytes (from the three donors used in d at 6–8 h post injection. Each cross represents the ratio calculated from all the monocytes tracked within a fish. Acquisition of the different conditions was performed in random order. Two-tailed p value < 0.05 (*), <0.005 (**), and <0.0001 (***). Statistical significance was determined by using a t test. Source data in  e are provided as a Source Data file