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Fig. S1
( A) Tg(nkx2. 3: mCherry;cmlc2: EGFP) embryos at 28 hpf with mCherry fluorescence (red) in the pericardium and EGFP fluorescence (green) in the heart. Single plane images showed that the GFP-positive myocardial cells were surrounded by the mCherry-positive cells. Scale bar, 50 μm. ( B) In situ hybridization of nkx2. 3 in whole mount embryos at 36 hpf (left panel, ventral view with anterior to the top). Sagittal section of the same embryo was shown in the right panel.
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