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Fig. 2
Mag and adhesion molecules of the paranodal axo-glial junction regulate myelin growth in zebrafish. a CNS myelin of 10/11dpf wild-type (wt), mag?/? and cntn1b?/? mag?/? fish. b Myelinated area at 10/11 dpf ( n?=?7?9; one-way ANOVA: p?<?0.0001). c Representative wt and mutant myelin sheaths at 10/11 dpf. d Sheath length at 10/11 dpf (means of 60 sheaths per animal, n?=?3, one-way ANOVA, p?<?0.0001). e? h Myelin sheath extension at 3 dpf (30?min interval, 15?16?h time lapse experiments). Asterisks in panel ( e) represent extending sheaths. Sheath length over time is shown in panel ( f) (15 sheaths from 3 animals). The fastest (magenta) and slowest (blue) extending sheath are highlighted. Extension rates ( h) were calculated from ( f) according to panel ( g). One-way ANOVA: p?<?0.0001. i Myelin sheaths of 3 dpf and 10 dpf cntn1b?/? mag?/? show differences in sheath intensity compared to wt. j Mean fluorescence intensities of 30 representative sheaths from 3 fish at 3 and 10 dpf, wt (left) and cntn1b?/? mag?/? (right). Unpaired two-sided t test: p?=?0.0473 (wt), p?=?0.0002 ( cntn1b?/? mag?/?). Data are presented as means?±?s.d. Scale bars, 10??m ( a, c, e), 5??m ( i). Source data are provided as a Source Data file