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Fig. 5
Generation of yap1b mutant alleles by CRISPR/Cas9 genome editing. (A) Schematic representation of the 136 bp and 84 bp deletions (shadowed sequence) generated in exon 2 of yap1b in medaka (see Materials and Methods). (B) The predicted Yap1b protein structure is depicted for the wild-type and mutant proteins. The yap1bΔ136pb deletion results in a frameshift mutation and premature stop codon, whereas yap1bΔ84pb results in a 28 amino acid deletion of the TEAD-binding domain. No phenotypic differences were observed when comparing wild-type (C,E,G) and yap1b mutant embryos (D,F,H) at stages 21 (C,D) and 24 (E-H). ov, optic vesicle; L, lens; nr, neural retina.