ZFIN Image: Chambers et al., 2019, Fig. S6

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Fig. S6

trm-/- mutant embryos exhibit normal proximal nephron segment pattern formation but have abrogated corpuscle of Stannius formation. A. WISH to evaluate proximal convoluted tubule (slc20a1a) and proximal straight tubule (trpm7) in WT and trm-/- at 24 hpf. Black arrowheads indicate the start and end of slc20a1a expression. Scale bar = 50μm. B. Quantification of absolute . lengths of slc20a1a expression. C. Quantification of absolute lengths of trpm7 expression. n = 3 for each control and test group. Measurements were compared by unpaired t-test. Data are represented as ± SD. N.S. = not significant. D. WISH to assess corpuscle of Stannius (stc1) development in WT and trm-/- at 48 hpf. Black line indicates stc1 expression domain. Scale bar = 20 μm.

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