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Fig. S4
Cranial bone and cartilage show no obvious defects at embryonic and juvenile stages. A. Alcian blue and Alizarin red double staining for cartilage and bone for 6 dpf scxa+/kg170 incross embryos, showing as flatmounts of the pharyngeal skeleton (ventral view, left) or neurocranium (dorsal view, right). B-C. 13 dpf scxa+/kg170;Tg(col2a1:mCherry) incross juveniles shown for live mCherry expression (ventral view, B) and Alcian blue and Alizarin red double staining (pharyngeal skeleton, ventral view, C, left and neurocranium, dorsal view, C, right). No dramatic differences are detected beyond normal variation.
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