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Fig. 4
Epigenetic profile of spermatozoa. (A) bars represent the percentage of global DNA methylation in spermatozoa of 3 pools of sperm from 5 control and 5 exposed fish each (n= 3). (B) bars represent the intensity of H3K9ac labelled with AlexaFluor®488 of 3 pools of sperm from 5 control and 5 exposed fish (n = 3); asterisks indicate significant differences (p < 0.05) when compared to control males. (C) confocal images of H3K9ac in spermatic nuclei (stained with DAPI); scale bar 5 µm.
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