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Fig. S3
(a) (1-2) Images representing the phenotypic differences between TRAF6+/+ and TRAF6−/− fishes at 50dpf. (3-4) Whole-mount in-situ Alizarin red staining of TRAF6+/+ and TRAF6−/− fishes at 50dpf. Boxes indicate area of enlargement on the right. White arrows indicate normal areas and black arrows indicate affected areas in vertebrae. Images were taken using a Nikon SMZ1500 stereomicroscope and a Nikon D5200 digital camera. Scale bar 2 mm. (b) mRNA expression levels of TRAF6 in bone tissue in 60 dpf wild-type zebrafish (n = 4 WT fish). Bar graphs show the means ± SEM.
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