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Fig. S3
A2a antagonism does not affect spinal motor nerve development. (A) In situ hybridization for wif1 in 54 hpf larvae treated 36 to 54 hpf with DMSO or 1.25 μM CGS-15943. Arrowheads denote MEP glia. SC, spinal cord; N, notochord. (B) Image of a 5 dpf larva treated from 30 hpf to 3 dpf with SCH-58261. Arrowhead marks a nkx+/sox10+ oligodendrocyte on the nerve, which is myelinating proximal segments of the nerve with nkx+/sox10+/MBP+ myelin (yellow brackets). A differentiated Schwann cell also myelinates a nerve segment with a nkx-/sox10+/MBP+ myelin sheath (red bracket). Scale bars, (A) 20 μm, (B) 50 μm.
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