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Fig. 7

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ZDB-IMAGE-190816-33
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Figures for Fogerty et al., 2019
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Fig. 7

Abnormal hair cells in mir-183/96lri69 and mir-183/96/182lri78 mutants. (a) Penetrance of abnormal swimming phenotype in mir-183/96lri69 mutants in a cross-sectional survey of fish in our colony at the time of manuscript preparation. mpf = months post fertilization. (b) Acute toxicity of gentamicin on lateral line neuromast hair cells, as determined by HCS-1 immunolabeling. Differences among genotypes within each treatment group are not statistically significant compared to WT controls (ANOVA with Dunnet’s multiple comparisons test). Each data point is the average of 3–5 fish. (c) Counts of the total number of stereociliary bundles per neuromast. There are no statistically significant differences (ANOVA with Dunnet’s multiple comparisons test). (d) The fraction of neuromast hair cells with abnormal sterociliary bundles (*p < 0.05, **p < 0.01, ANOVA with Dunnet’s multiple comparisons test). For (d-e), sample sizes are: WT, 14; lri70, 4; lri60, 4; lri61, 4; lri69, 8; lri79, 8; lri81, 4; lri78, 13. (e) Representative images of neuromast stereociliary bundles, stained with phalloidin. Arrows indicate small or disorganized bundles that we scored as abnormal.

 

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