Fig. 5

Fig. 5

In vitro holding of the long mature actinotrichia by the basal keratinocytes from two reporter lines. (A) Schematic diagram of the mix-culture experiment using two types of reporter zebrafish. Scale bar is 50?μm. (B–J) The cultured basal keratinocytes in vitro from the larvae of and1:mCherry-CaaX and and1:GFP-CaaX. (B) White arrow indicates the actinotrichium in the basal keratinocyte expressing mCherry-Caax. (C) White arrowhead indicates the actinotrichium in the basal keratinocyte expressing GFP-CaaX. (E) White arrow indicates the actinotrichium protruded from a basal keratinocyte were enveloped by a mCherry-CaaX-expressing basal keratinocyte. (F) White arrowhead indicates GFP-CaaX-expressing basal keratinocyte. (D and G) Merged image of (B and C) and (E and F), respectively. Two adjacent basal keratinocytes share the same actinotrichia. Scale bar in (B?−?G) is 50?μm. (H–J) Time-lapse images of the plasma membrane enveloping the actinotrichium. White arrowheads indicate the actinotrichium enveloped by two adjacent basal keratinocytes. Scale bar in (H–J) is 20?μm.