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Fig. 2
A) Example of a zebrafish with a GFP positive heart. B) Copy number analysis of integrated human PTDSS1, GFP and mCherry by q-PCR on gDNA isolated from individual GFP- fluorescing zebrafish. C) Comparison of DNA copy number (top panel) and RTq-PCR expression analysis (bottom panel) for individual zebrafish where DNA and RNA were extracted from the same samples. D) Comparison of endogenous zebrafish ptdss1 with human PTDSS1 showing significantly higher levels of endogenous transcripts over WT and mutant transgene transcripts (p=0.01 and 0.008 respectively).Expression analysis in transgenic zebrafish.
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