Fig. 2
HCprs undergo long heterogenous cell cycles. (A) Montage of the HCpr cell cycle. Trβ2:tdTomato (HCpr, green) and PCNA-GFP (nuclei, magenta). White arrowheads indicate the tracked cell. The OPL is indicated by a dotted white line. Scale bar: 10 µm. Bottom panels show Trβ2:tdTomato and PCNA-GFP individually. Scale bar: 5 µm. (B) HCpr cell cycle phases occur at different positions in the INL. Trβ2:tdTomato (HCpr, green) and PCNA-GFP (nuclei, magenta) are shown. White arrowheads indicate the cell that was followed. The OPL is indicated by a dotted white line. Scale bar: 10 µm. Bottom panels show Trβ2:tdTomato and PCNA-GFP individually. Scale bar: 5 µm. (C) Distribution of HCpr cell cycle length. Line indicates the mean. (D) Scatter plot of HCpr cell cycle length versus HCpr position shows a lack of strong dependence. Regression analysis was carried out using Pearson correlation (r). (E) Distribution of HCpr cell cycle phase lengths: S, G2 and M. Lines indicate the means.