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Fig. 3
The anti-apoptotic effect of SB on 6-OHDA-induced neurotoxicity in SH-SY5Y cells: SH-SY5Y cells were pretreated with 0.1 nM SB for 1 h and then challenged with 20 μM 6-OHDA for 8 h in the control, 6-OHDA, 6-OHDA plus SB, and SB alone treatment groups. (A) TUNEL staining. White arrows indicate apoptotic cells (scale bar = 100 μM); (B) Quantification of apoptotic cells in each treatment group; (C) Western blotting showing induction of cleaved caspase-3 protein; (D) Quantification of relative density of cleaved caspase-3 protein from Western blotting. Data are presented as mean ± SEM, and each value represents the mean of three replicates and three samples. *significantly different from the control group; # significantly different from the 6-OHDA group.