IMAGE

Fig. 4

ID
ZDB-IMAGE-190807-4
Genes
Source
Figures for Talbot et al., 2019
Image
Figure Caption

Fig. 4

met is required for normal MMP migration. (A-F) Confocal projections of six1b:lyn-GFP (green) and mylpfa:mCherry(magenta) transgene expression in embryos fixed at indicated stages. Compared with DMSO-treated controls, treatment with the Met inhibitor SGX523 causes reduced migratory streams at 36 and 48 hpf and smaller MMP-derived muscles at 72 hpf. (G-J) Confocal projections of phalloidin-labeled wild-type and met mutant embryos at 96 hpf, false-colored to show muscle identity using color code described in Fig. 1. The fin and PHM muscles are shown in lateral view in G and H and the more anteriorly located SHM is shown in ventral view in I and J. Insets in E-H show confocal sections through the fin to distinguish AbFM and AdFM muscles. In met mutants and SGX523-treated embryos, the AbFM is more severely affected than the AdFM and in this example the AbFM is lost entirely. (K) Box plots of 96 hpf wild-type and met mutant MMP-derived muscle measurements. For all plots, WT/Het values are shown in gray and met mutant values are shown in brown. Asterisks indicate significant differences (P<0.01), determined by Tukey–Kramer HSD comparisons after one-way ANOVA; n.s. indicates not significant. See Materials and Methods for statistical details. Measurements were taken on a total of 31 mutants and 29 WT/Het siblings from three separate experiments. Arrowheads are as described in Fig. 1 legend. Scale bars: 100 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development